Abstract

Workshops

Day 1: Wednesday, May 18　18:10-18:30　Room A (Orbit Hall)

1A-W1-1810　　PDF

Matrix effect and LC separation for LC/MS analysis

(¹Shimadzu, ²Nihon Waters, ³Agilent, ⁴AB SCIEX, ⁵Bruker Daltonics, ⁶Thermo Fisher)
^oTaku Tsukamoto¹, Shinichiro Kawabata¹, Futoshi Sato², Tatsuya Ezaki², Takeshi Serino³, Takeshi Shibata⁴, Yoshihiko Takinami⁵, Masayuki Kubota⁶

Given the growing LC/MS performance, ability and usability, LC/MS is becoming popular in various fields as a highly sensitive and selective analytical method to detect very small amount of target compounds in samples including complex matrices without complicated sample preparations. Though easy sample preparation relying on LC/MS selectivity increase laboratory efficiency, validation for sample matrix effect is necessary to assure accuracy and precision of analytical result.
Well known matrix effects are ion-enhancement and ion-suppression at MS ion source but matrices also affect separation at LC and cause peak shape distortion and retention time shift. To avoid these matrix effect, sample dilution and SPE is popular, however in some cases changing LC analytical condition or separation mode also decrease matrix effect dramatically.
In this workshop, we introduce about a example of matrix effect improvement for pesticide analysis in food sample.