1A-W1-1750 PDF
SPE method development to reduce matrix effect on quantitative analysis with LC/MS
Analytical scientists are faced with issue caused by complex sample matrices. It can be seen on quantitative analysis with LC/MS. For proper MS signal response, the compound ion must be allowed to form properly. In cases where the formation of the compound ion is suppressed by interferences in the sample matrix, the signal strength is greatly diminished. As a solution, SPE is often used for the selective removal of interferences. Solid-phase extraction (SPE) method development starts with knowledge of the sample matrix-the nature and relative concentration of the analytes of interest and the potentially interfering endogenous compounds. The right sorbent and solvents are selected for load, wash, and elute steps, resulting in the creation of rugged, reliable, and selective protocols. In this workshop, we introduce about the protocol to reduce ion suppression or enhancement on quantitative analysis.