Abstract

Oral Sessions

Day 1: Wednesday, May 18　16:45-17:05　Room A (Orbit Hall)

1A-O1-1645　　PDF

Can mass spectra shed a new light on the twilight zone of the database search?

(¹Kyoto Univ., ²Kyoto Univ., ³DBCLS, ⁴Niigata Univ., ⁵Niigata Univ., ⁶Kyushu Univ., ⁷Kumamoto Univ.)
^oAkiyasu Yoshizawa¹, Tsuyoshi Tabata², Yuki Moriya³, Shin Kawano³, Shujiro Okuda⁴, Tadashi Yamamoto⁵, Masaki Matsumoto⁶, Daiki Kobayashi⁷, Norie Araki⁷, Naoyuki Sugiyama², Susumu Goto¹, Yasushi Ishihama²

The database search protocol is the mainstream for current proteomics analyses. However, the results by this protocol usually contain false positive hits, requiring careful interpretations of the results. Based on this fact, we are now developing a methodology to remove these "results in the twilight zone," namely unreliable results that can be false positives, from the search results, in order to develop an integrated proteome database "jPOST," which has been developed since last year. For this methodology, we are focusing the features of obtained mass spectra to select reliable spectra equivalent to manual inspection. In this presentation, we will introduce the development and the analyses for jPOST and discuss the improvement of database search results by the careful analyses of mass spectra.