1A-O1-1425 PDF
Frontier in biological glycoprotein analysis by mass spectrometry
Analysis of biological glycoproteins by mass spectrometry is challenging because of the heterogeneity of glycosylation and ionization suppression by co-existing peptides and surfactant agents. To overcome these problems we have developed glycopeptide enrichment methods and a high-throughput glycoproteomic approach using endoglycosidases. These methods are successfully used in the glycosylation analysis of serum, ER and membrane glycoproteins.