3P-46 PDF
High Throughput simultaneous analysis of 39 amino acids in various foods without derivatization using LC-MS/MS
As amino acids in foods are essential components of nutrition and taste, it is expected to develop an easy and efficient analytical method. Amino acids are high polar compound, so it needs their derivartization or addition of ion pair reagent in mobile phase in order to separate them by reversed phase mode. And in the case of hydrophilic interaction chromatography, it is not enough for separation of isomers or comprehensive analysis.
In our previous study, we developed a simultaneous analysis method of 20 amino acids by LC-MS/MS with mix-mode column (ion exchange and normal-phase) without derivartization. This time, we tried to enhance the number of analytical amino acids (39 amino acids), and detect them in various foods with high sensitivity.