3P-35 PDF
Large-scale profiling of protein kinases by targeted phosphoproteomics
Phosphorylation is an essential regulatory mechanism to control various cellular functions. Quantitative phosphoproteomics is a powerful tool to monitor the entire map of cellular phosphorylation of proteins, in order to understand the comprehensive biological functions. In this study, we targeted kinase-derived phosphopeptides including phosphosites which are known to regulate the kinase activity. By quantifying these phosphopeptides using parallel reaction monitoring LC-MSMS, we successfully profiled 50 kinases in human cancer cells treated with various molecular-targeting drugs.