3P-22(3C-O1-0930) PDF
Localization analysis of natural toxin via mass spectrometric imaging
It is well known that Potato (Solanum tuberosum L.) is one of major agricultural crops and, produces α-solanine and α-chaconine (Fig. 1) as natural toxin on budbreak. These are categorized as glycoalkaloids and, induce gastro-intestinal and systemic effects in human as well as livestock. After budbreak, we often remove a germ to eat in safety, however sometime we do not remove a periderm as nutrition under the wrong impression. Previous studies have revealed and concerned the increasing of these toxin at periderm after budbreak and exposure the sunlight. However, it is not common knowledge and involves food intoxication. In order to facilitate increased direct visualization of biomolecules during biological analysis, mass spectrometry is usually used to distinguish individual molecules from each other. Mass spectrometric imaging (MSI) enables simultaneous detection of multiple analytes even in the absence of the target-specific markers such as antibodies.
In this study, we visualized the localization of α-solanine and α-chaconine in Potato to easily understand food safety by MSI. In addition, Nano-Particle Assisted Laser Desorption/Ionization (Nano-PALDI) was also used to achieve high-spatial resolution MSI.