3C-O1-1010 PDF
An Investigation Into Multi-Model Tissue Imaging on a Single Section by DESI and MALDI TOF Mass Spectrometry
Desorption electrospray ionisation (DESI) is an established technique in the field of mass spectrometry, and has been used for tissue imaging (MSI). DESI results are of very high-quality, for lipids and small molecules compared to the more established matrix assisted laser desorption ionisation (MALDI) imaging technique. An additional advantage of DESI is that it can be performed without further sample preparation, such as matrix deposition. Using optimized gas and solvent flow rates, the DESI technique does not damage the tissue surface.
Here we present work showing the potential to analyse a single tissue section by DESI imaging with a subsequent analysis by MALDI imaging of the same tissue section.