3B-O2-1545 PDF
Imaging mass spectrometry of liver regeneration after partial hepatectomy in mice targeting primary bile acids and nucleotides
Introduction: The liver plays a major role in metabolism with numerous functions with dynamic structural and functional heterogeneity, known as “metabolic zonation”. In this study, we applied MALDI (matrix-assisted laser desorption ionization) IMS (imaging mass spectrometry) into the analysis of the partial hepatectomy (PHx) model in mice.
Methods: Normal ICR mouse underwent 70% PHx. At specified timings, the remnant liver was removed, followed by heat stabilization by Stabilizor T1 (Denator). 9-Aminoacridine was applied by airbrush sprayer on 10μm thick frozen liver sections, and the MALDI measurement and image analysis were carried out on an iMScope (SHIMADZU Corporation).
Preliminary data: MALDI IMS showed the presence of many signals including nucleotides and taurocholic acid (TCA). The tissue preparation with heat stabilizer allowed us to observe more signals with longer duration. Above all, TCA succeeded in visualizing the hepatic lobules clearly by encircling with its signals in the interlobular portal triads. Additionally, remarkably high signals of TCA were detected also in the gallbladder wall. Furthermore, we analyzed some peaks, which were specifically detected after PHx in comparison to the normal liver.
Novel aspect: Applications of MALDI IMS into the hepatectomy model provided a clue to understand liver regeneration with temporospatial information.