2P-38 PDF
Application of metal-free ODS column for glycerophospholipids analysis by LC/MS/MS
Among all phospholipid classes, phosphatidic acid and phosphatidylserine tend to elute as broad peaks under the reversed phase LC conditions. In this study, we aimed to detect sharp peaks of phospholipids by using metal-free ODS column and optimizing LC/MS/MS system. PA (500 pg) and PS (500 pg) were not eluted using a stainless steel column under optimized conditions. By contrast, they were eluted as sharp peaks using a metal-free column. We suggest that they were adsorbed to chromatographic tube and frit made from stainless steel.