2P-36 PDF
Concentrations of α-pinene in blood, urine and saliva from forest walkers
Most vegetation emits an array of volatile organic compounds (VOCs). The major components of conifer-derived VOCs are monoterpenes (MTs) such as α-pinene. α-pinene dominates conifer trees emissions and research investigations have raised concerns regarding the physiological effect of α-pinene . However, no quantitative study is available regarding transfer of MTs from conifer trees to subjects. Venous blood, urine and saliva were obtained from 4 healthy volunteers after forest walking. MTs in the forest atmosphere and in samples were adsorbed on solid phase micro extraction (SPME) fibers for 60 min and identified by GC-MS (JEOL JMS T100 GCV ). The concentrations of α-pinene in the blood, urine and saliva were determined by comparison with standard curves prepared in the same concentration range after the addition of α-pinene. Concentrations of α-pinene in the blood, urine and saliva from walkers after walking were 5~10, 0.5~2 and below 0.5 nM, respectively