2P-04 PDF
Differential Ion Mobility for Separation of Reduced Intact Monoclonal Antibody
The mass measurement of intact proteins and their heterogeneity is important for organizations that wish to present a consistent and reliable protein drug product. Methods for the analysis of intact proteins and antibody-drug conjugates by LCMS are often fast and simple, with the expectation that they provide a rapid answer confirming the molecular weight and/or drug-antibody ratio. Differential ion mobility mass spectrometry (DMS) separates ions based on their dipole moment instead of by m/z. This provides an orthogonal technique for improving data quality in the quantitation and characterization of challenging samples requiring advanced analytical selectivity. We present here the capability to separate reduced antibodies using DMS on a modified Qq-TOF mass spectrometer. The implications of this work can save days of method development time per protein, and have a better chance of accelerating assays that require intact protein molecular weight determination.