1P-27 PDF
MALDI imaging mass spectrometry of N-linked glycans from formalin-fixed paraffin-embedded mouse brain.
Recent developments in spatial proteomics have paved the way for retrospective in situ mass spectrometry (MS) analysis of formalin-fixed paraffin-embedded (FFPE) clinical tissue samples. This type of analysis is commonly referred to as matrix-assisted laser desorption/ionization (MALDI) imaging. Recently, formalin-fixed paraffin-embedded MALDI imaging analysis was augmented to allow in situ analysis of tissue-specific N-glycosylation profiles. In the present study, we outline an improved robust sample preparation method for N-glycan MALDI imaging, which uses in situ PNGase F-mediated release and measurement of N-linked glycans from sections of formalin-fixed mouse brain. This MALDI-IMS workflow has the potential to be applied to any FFPE tissue block or tissue microarray to enable higher throughput analysis of the global changes in N-glycosylation.