1D-O1-1625(1P-11) PDF
Structure-property Relationship and Strategic Synthesis of Matrix for MALDI
Recenly, matrix-assisted laser desorption/ionization (MALDI)-mass spectrometry (MS) has been used not only for biopolymer analysis but also for low-molecular-weight metabolite analysis because it is a highly sensitive, high-throughput, and low sample-consuming technique compared with other conventional analytical platforms such as LC-MS or GC-MS. More importantly, MALDI-MS can only achieve direct tissue analysis that requires both measurements of solid-state sample and non-preseparation processes. In MALDI-MS analysis, the molecular coverage of detectable compounds is strongly associated with the molecular species of the matrix. At present, little is known about matrix capable of ionizing all compounds, and the detectable coverage is very narrow. Therefore, application of MALDI-MS for low-molecular-weight compound analysis is still limited to not many interesting compounds but analyzable ones using commercially available matrices.
Currently, it has been reported that the MALDI-MS-system with 9-aminoacridine (9-AA) as a matrix improves the sensitivity of low-molecular-weight metabolite analysis in negative ionization mode. However, even by using 9-AA as a matrix, there are numerous low-molecular-weight metabolites that are hardly ionized. Herein, we report structure-property relationship between novel synthesized matrix and ionization efficiency of representative low-molecular metabolites in negative ionization mode MALDI-MS analysis.