1C-O2-1740(1P-15) PDF
Utilization of LipidBlast in silico tandem mass spectrometry spectral library for lipid annotation
Global profiling of polar lipids constitutes a core technology in a variety of applied research areas. Liquid chromatography-mass spectrometry (LC-MS) analysis with mass spectral library search is exemplary, but structure identification or annotation of lipid species is hampered by the limited availability of authentic standard compounds even when used in combination with high-resolution tandem mass spectrometry. As an alternative to conventional lipid identification pipelines, we employ LipidBlast in silico tandem mass spectrometry spectral library. Here we present the lipid annotation in Euglena gracilis using LipidBlast. We successfully demonstrated profiling of anoxia-induced phospholipid metabolism in E. gracilis by means of LipidBlast. The lipid annotation pipeline using such a virtual spectral fragmentation library offers a unique option for diverse research areas.