3P-34 PDF
Analysis of metabolite in urine without any separate sample preparation
When analyzing metabolite in urine using Liquid Chromatography and Mass Spectrometry, in general, the urine sample is needed to be pretreated separately by Solid-Phase Extraction (SPE), dilution, etc. In order to avoid such tedious pretreatment procedure, we tried to combine on-line SPE-LC system and Mass Spectrometry so that the sample preparation and separation analysis can be implemented at one time. As a result, we succeeded in detecting some metabolites in urine without any separate sample preparation.