Abstract

Poster Presentations

Day 3: Friday, May 16　　Poster Room(Gekko)

3P-24　　PDF

Determination of α-pinene concentration in the blood and urine using SPME/GC/MS

(¹Asahikawa Med. Univ., ²Asahikawa Med. Univ., ³Asahikawa Med. Univ.)
^oHiroaki Akutsu¹, Kazuhiro Sumitomo², Shin Kukita², Yoshiaki Sato², Shusei Fukuyama², Shinobu Osanai², Hiroshi Funakoshi¹, Naoyuki Hasebe³, Masao Nakamura²

Introduction
We have reported that forest walking results in the modification of autonomic activity and a decreasing blood pressure. Conifer trees produce volatile organic compounds (VOCs) such as isoprenoids and terpenoids (α-, β-pinene, camphene, limonene and bornyl acetate). Anti-inflammatory and anti-cancer effect of essential oils containing geraniol or limonene have been reported¹⁾. We have reported the identification of conifer-derived VOCs in the atmosphere of conifer forest and in blood and urine from the conifer forest walkers²⁾. In this presentation, we have tried the determination of α-pinene concentration in the blood or urine from the forest walkers.

Methods
Stock solution containing 100mM of α-pinene was prepared in DMSO. Standard α-pinene solution was diluted to 500pM, 5nM, 50nM and 500nM to build the calibration curve. Sample vial was preheating 20 min at 55°C, then headspace adsorption 20min at 55°C by solid phase micro extraction (SPME). The samples were then analyzed by GC/MS.

Results and Conclusion
Linearity of the calibration curve was ranged from 500pM to 500 nM. The concentrations of α-pinene in the blood and urine after forest walking were 3-7nM and 0.1-6nM, respectively.

References
1) P.L.Crowell. J.Nutr.129,775S-778S(1999)
2) K.Sumitomo, el al., 61th MSSJ Annual Conference, 2P-49 (2013)