2P-26 PDF
Development of in vitro screening endocrine disruptor by steroid profiling
The aim of this study was to apply metabolic profiling to phenotype analysis of cells exposed to chemicals, and to develop a system to evaluate endocrine disruptors by multimarker profiling based on chemical exposure-induced endogenous metabolite changes. First, we constructed a platform to simultaneously analyze steroids in steroidogenesis pathways. To construct a versatile evaluation system, gas chromatography/mass spectrometry (GC/MS), which is known for its versatility, was used in this analysis. Seventeen steroids were simultaneously analyzed under optimized preparation and GC/MS conditions. Next, we constructed a screening system for endocrine disruptors. Following the protocol of OECD TG 456, the human adrenocortical carcinoma cell line H295R exposed to forskolin and prochloraz was used as a positive control. Steroid profiles were obtained successfully.