2P-23 PDF
LC/MS analysis of the modified products of GSH in mouse melanoma cells exposed to CSE
Cigarette smoke extract contains many oxidants and induces injury of various cells. We have studied the identification of active ingredients in water extracts of cigarette smoke (CSE). In a previous study, we determined that glutathione in mouse melanoma cells was modified by methyl vinyl ketone (MVK), which is an α, β-unsaturated carbonyl compound. Glutathione (GSH) is a major detoxication agent in cells. In this study, to clarify the time required for the reaction, we exposed mouse melanoma cells to CSE and used LC/MS to measure changes in GSH levels and its modified products in the cells. We found that the concentration of GSH rapidly decreases within 5 minutes after exposure and the concentration of reaction products (<I>m/z<I> 378 and <I>m/z<I> 366) increases. The peak of <I>m/z<I> 366 was estimated as reduction product of GSH-adduct with acrolein (ACR).