2P-03 PDF
Development of Imaging Mass Spectrometry Using Laser Desorption/Ionization for Analysis of Transcutaneous Drug Delivery
Transcutaneous vaccine delivery, which is an easy-to-use, low-invasive, and painless method, has the potential to overcome certain issues associated with injectable vaccination. Adjuvants, which are used for enhancement of immune responses with vaccine, have not been developed for the transcutaneous vaccine. Therefore, it is desired to discover an effective and safe adjuvant available for transcutaneous delivery. In vivo imaging technology can greatly contribute to kinetic analysis of adjuvants in the skin to predict their effect and safety. The purpose of this study is to image the adjuvants administrated transcutaneously in the mouse skin tissue sections by Imaging Mass Spectrometry (IMS) using MALDI (Matrix assisted laser desorption/ionization) method, which is unnecessary to label and can measure the distribution of the object. This study develop the protocol of IMS. Experimentally, FSL-1 using DHB has the most ionization efficiency. Lipids were detected by IMS. Then, FSL-1 with a volume of 40 μL at a concentration of 300 μg/mL was administrated in mouse skin tissues by injection, and the ionized FSL-1 was detected. In future work, spatial resolution is developed by using MULTUM-IMG.