1P-05 PDF
Effect of N-terminal Derivatization on Discrimination between Leucine and Isoleucine in Peptides by High-energy CID MS/MS Analysis
High energy collision induced dissociation (HE-CID) is effective to discriminate between isobaric Leu and Ile in de novo sequencing of peptides. In this study, we derivatized the N-terminus of a peptide by using several derivatization reagents, which have the high proton affinity, and compared their effects on generation of d-ions necessary for Leu/Ile discrimination under HE-CID conditions. Among three tested reagents, 4-(guanidinomethyl)benzoic acid was the most effective for generation of d-ions.