1A-O1-1420 PDF
Proteomics and peptidomics analyses of serum/plasma by mass spectrometry demand comprehensive and careful pretreatment protocols
Serum and plasma contain thousands of different types of proteins and peptides, which can provide valuable information about the numerous processes that take place within the body. However, detailed analyses of proteins and peptides in serum and plasma are still challenging because of the presence of many high-abundance proteins, the large dynamic range of protein and peptide concentrations, rendering the extremely high complexity of the serum/plasma proteome. Therefore, we have to optimize both pretreatment strategies and analysis methods. Furthermore, verification and validation studies are necessary to clarify complicate biological process and to develop disease biomarkers from human serum/plasma because of considerable individual variability. In this symposium, I will talk about our challenges to develop biomarkers and find out maximum information about important biological processes from a drop of blood.