ポスター発表
- 第3日 5月13日（水） P会場（1008/09）
-
3P-13 PDF
ヒト血清中アビラテロンおよびその代謝物のLC/MS定量における条件検討
Abiraterone (Abi) is used for the treatment of castration-resistant prostate cancer. Abi is metabolized to a more active Δ4-abiraterone (D4A) than Abi by 3β-hydroxysteroid dehydrogenase and further D4A is metabolized to keto-5α- and keto-5β-Abi by 5α- and 5β-reductases, respectively. Furthermore, keto-Abi’s are metabolized to their hydroxy metabolites, 3α-OH-5α-Abi and 3β-OH-5α-Abi, and 3-keto-5β-Abi and 3β-OH-5β-Abi. In this study, we examined the pretreatment and separation conditions for Abi and its seven metabolites in human serum for their quantification. A Zorbax Eclipse plus C18 column showed the best separation for them among the C18 columns tested. A silica-based Strata-Si solid-phase extraction column gave the best recovery and the lowest matrix effect among the pretreatment methods used. The analytes were quantified using extracted ion chromatograms obtained by Q-TOF mass spectrometer under the optimal pretreatment and separation conditions.