オーラルセッション
- 第3日 5月13日（水） 15:10～15:30 A会場（特別会議場)
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3A-O13-1510 PDF
イオンクロマトグラフィー四重極オービトラップ型高分解能質量分析 (IC/HRMS/MS) を用いた次世代メタボローム分析法の開発
Almost all intermediates of essential metabolic pathways to maintain biological activities are cationic, zwitterionic, or anionic metabolites. Therefore, a profiling for these ionic metabolites provides valuable insight towards understanding metabolic regulation. To date, we have developed methods for the analyses of anionic metabolites and cationic metabolites, respectively, using ion chromatography high resolution tandem mass spectrometer (IC/HRMS/MS). However, in conventional IC system, many of zwitterionic metabolites such as amino acids and their derivatives are removed by a process that uses suppressors to convert the high salt eluent to pure water (i.e., desalting). The aim of this study is to develop an advanced analytical system for wide-range metabolomic analysis of cationic, zwitterionic, and anionic metabolites by IC/HRMS/MS with novel cationic and anionic suppressors.