オーラルセッション
- 第2日 5月12日（火） 14:30～15:10 A会場（特別会議場)
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2A-O6-1430 PDF
Integrated Analysis of Multiomics of Diffuse Gastric Cancers in Young Korean Population
Investigating the molecular profile of the disease is essential for understanding its role in the etiology of the disease. Advances in multiomics technologies, including next-generation sequencing and mass spectrometry, enable multiomics analysis of target diseases. In this presentation, we report proteogenomic analysis of diffuse gastric cancers (GCs) in young population. Multi-dimensional proteomic analyses provide different layers of protein information, including protein abundance and post-translational modifications. Here, we report an integrated analysis of protein expression, phosphorylation, and N-glycosylation by serial enrichments of phosphorylation and N-glycosylation (SEPG) from the same tissue samples. On average, the SEPG identified 142,106 unmodified peptides of 8,625 protein groups, 18,846 phosphopeptides (15,647 phosphosites), and 4,019 N-glycopeptides (2,634 N-glycosites) in tumor and adjacent normal tissues from 80 gastric cancer patients. Interestingly, phosphoproteome data elucidated signaling pathways associated with somatic mutations based on mutation-phosphorylation correlations. Moreover, correlations between mRNA and protein abundances provided potential oncogenes and tumor suppressors associated with patient survival. Therefore, our proteogenomic analysis provides additional information beyond genomic analyses, which can improve understanding of cancer biology and patient stratification in diffuse GCs.