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第2日　5月12日（火）　10:25～10:45　A会場（特別会議場)

2A-O4-1025　　PDF

DIUTHAMEを用いたアルツハイマー病脳イメージング質量分析法

(¹同志社大生命医・ ²浜松ホトニクス・ ³ブルカージャパン・ ⁴都健康長寿医セ研・ ⁵光産業創成大学院大)
^o外山友美子¹・ 武笠結天¹・ 新谷祈¹・ 大村孝幸²・ 小谷政弘²・ 田代晃²・ 韮澤崇³・ 角田伸人¹・ 村山繁雄⁴・ 内藤康秀⁵・ 池川雅哉¹

Neuropathology of Alzheimer’s disease (AD) is characterized by the accumulation and aggregation of Amyloid β (Aβ) peptides into extracellular plaques of the brain. Increasing lines of evidence support a direct involvement of cholesterol in AD pathogenesis as well. For the rapid and reproducible analysis of multifactorial autopsy brains, here we adopt a novel matrix‐free laser desorption/ionization method, Desorption Ionization Using Thorough Hole Alumina Membrane (DIUTHAME) in combination with conventional MALDI-IMS on human autopsy brain tissues to obtain a comprehensive molecular mapping.
Frozen tissue sections were cut on a cryostat at a 20 µm thickness onto ITO glass slides. Spectra were acquired using the rapifleX MALDI Tissuetyper, whereas ions were detected with spatial resolution of 50 µm.
We have succeeded in obtaining low molecular imaging with DIUTHAME system for the brains from AD in contrast with non-pathological aged subjects. In order to gain sensitivity, we utilized TM-sprayer for the hydration of the surface of the DIUTHAME cassette. From AD and non-pathological brain section, m/z at 147 for grey matter dominant and m/z at 184 for white matter dominant. These can be annotated to cholesterol fragment and phosphatidylcholine fragments, respectively.