ポスター発表
- 第1日 5月11日（月） P会場（1008/09）
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1P-22 PDF
ネイティブ MSにおけるTrapped Ion Mobility Spectrometry（TIMS）の活用
It is important to understand how no-covalent protein-protein complexes assemble and interact for the advancement of structural biology. Although X-ray crystallography is the gold standard for resolving atomic structures of protein complexes, electrospray mass spectrometry in native condition is also known as another tool for characterizing non-covalent protein complexes.
Bruker’s timsTOF Pro, a high resolution QTOF mass spectrometer coupled with trapped ion mobility spectrometry (TIMS) device, has emerged as a powerful tool for shotgun proteomics due to its high speed and high resolving powers in both ion mobility and m/z.
Recently I applied this new instrument to analyze no-covalent protein-protein complex in native mass spectrometry condition using the standard ESI source and newly developed offline nano ESI source.
In this presentation, I would like to show the results acquired by timsTOF Pro.