ポスター発表
- 第1日 5月11日（月） P会場（1008/09）
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1P-18 PDF
単糖分析に同位体希釈を応用した糖鎖絶対定量法の開発
Monosaccharide analysis was applied to isotope-dilution mass spectrometry to accomplish absolute quantification of N-linked oligosaccharide. The hydrolysis condition is examined using six N-linked oligosaccharides and determined to be 110 °C for 4 hours with 2 M trifluoroacetic acid. Under the optimized condition, the measured concentrations of each constituted monosaccharid showed a good agreement each other and the highest result among the measured monosaccharides, which represented complete hydrolysis of the analyte oligosaccharide. This method allows accurate quantification for oligosaccharide.