日本質量分析学会 第68回質量分析総合討論会会

演題概要

ポスター発表

第1日 5月11日(月)  P会場(1008/09)

LC-MSによる脳内神経ペプチドの極微量定量解析における問題点とトラブルシューティング

(サントリー生科財団)
o山崎尭嗣山垣亮

Quantitative analysis of neuropeptide Y (NPY) was developed by LC-orbitrap mass spectrometry (LC-MS). NPY was so sticky that the carry-over in LC system and non-specific adsorption occurred in the LC-MS system. The metal-free pipes were used in LC system and the optimized matrix solvents with peptide fragments, acids, organic solvents and so on were applied to the sample solvent to hinder the non-specific adsorption of NPY.