オーラルセッション
- 第1日 5月11日（月） 16:00～16:15 C会場（1101/02）
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1C-O3-1600（1P-38） PDF
MALDI-ISDにおけるペプチドからの複数水素の脱離とラジカル誘導分解
The formation and radical-directed dissociation (RDD) of multiple hydrogen abstracted peptide cations [M+H-mH]•+ has been examined using MALDI In-source decay (ISD) with four different dinitro-substituted matrices. The MALDI-ISD of peptides using 3,5-dinitrosalicylic acid (3,5-DNSA) and 3,4-dinitrobenzoic acid (3,4-DNBA) as matrices resulted in multiple hydrogen abstraction from protonated peptide [M+H]+ and production of fragment [a]+ ions, i.e., [M+H-mH]•+ and [a-mH]•+ (m=1-8). The intensity of [an]+ series ions generated using 3,5-DNSA as matrices rapidly decreased with increasing residues number n, while that of the [an]+ ions with 4-nitro-1-naphthol (4,1-NNL) decreased smoothly.