Abstract

Poster Presentations

Day 3, May 17（Fri.）　　Room P (Multi-purpose Hall)

3P-08　　PDF

Lipid and Fatty Acid Isomer Analysis Using a Travelling Wave Cyclic Ion Mobility Separator

(¹Nihon Waters, ²Waters - Wilmslow, ³Waters - Milford)
^oThanai Paxton¹, Kenji Hirose¹, Michael Mccullagh², Martin Palmer², Giorgis Issac³, Russel J. Mortishire-smith², James I. Langridge², Johannes P. C. Vissers²

Lipids represent a diverse group of biomolecules that have an essential role in structural, storage and signalling processes in living systems. Class separation is readily achieved using chromatographic and MS based identification techniques; however, the analysis remains challenging due to the chemical structure diversity and isobaric nature of lipids. The addition of ion mobility (IM) to lipidomic workflows enhances separation and improves isomer resolution. A novel cyclic ion mobility-enabled quadrupole time-of-flight (Q-cIM-oaToF) mass spectrometer geometry has been characterized and successfully applied to the IM separation of isomeric, mono-unsaturated FAs. The required IM resolution was found to be a function of chain length. Shorter, structurally more rigid and compact FAs were discriminated at reduced resolution, as could longer chain mono-unsaturated FAs as a result of partial chain back-folding. Following IM separation, isomeric lipids were successfully CID fragmented and identified.