日本質量分析学会 第67回質量分析総合討論会

Abstract

Oral Sessions

Day 3, May 17(Fri.) 15:05-15:25 Room E (201)

13C-Metabolic Flux Analysis of Non-Epithelial Cancer Cells Using Mass Spectrometry

(Osaka Univ.)
oTomoki Shima, Masaharu Maruyama, Nobuyuki Okahashi, Fumio Matsuda

The 13C-metabolic flux analysis (13C-MFA) using mass spectrometry was attempted to investigate metabolic flux distributions in the non-epithelial cancer cells including YKG-1 (human glioma) and HT-1080 (human sarcoma). A time course analysis of cell numbers, medium components and 13C-labeling kinetics of the intracellular metabolites confirmed that 13C-MFA could be applied for the non-epithelial cancer cells. Flux distributions in the central carbon metabolism suggested that there was variation in the NADPH method among the cancer cell lines.