日本質量分析学会 第67回質量分析総合討論会

Abstract

Oral Sessions

Day 3, May 17(Fri.) 10:50-11:10 Room E (201)

Nanoparticle Assisted Laser Desorption/Ionization for Imaging Mass Spectrometry of a Single Cell

(1Osaka Univ., 2Kansai Univ.)
oBrijesh Sharma1, Jun Aoki1, Yukihiro Miyanaga1, Hideya Kawasaki2, Michisato Toyoda1

Single cell analysis is very useful in knowing cellular functions and their response to external stimuli. Recently, Surface-assisted laser desorption/ionization mass spectrometry (SALDI-MS) using inorganic nanoparticles or nanostructured surfaces is becoming popular for the LDI-MS analysis of small molecules as an organic matrix-free approach. Compared to MALDI-MS, SALDI-MS affords certain advantages, such as low background noise, high salt tolerance, better shot-to-shot reproducibility. The use of an organic matrix in MALDI Imaging MS can limit the spatial resolution because of the large matrix crystal size formed by the MALDI matrix. Moreover, the spatial resolution of a conventional MALDI instrument is limited by the laser focus diameter to 10-100 m which is inadequate for cellular-scale observation. To resolve this problem, we will use a stigmatic type imaging mass spectrometer developed in our laboratory in which the spatial resolution is not dependent on the laser focus diameter and we can achieve the spatial resolution in the sub-micron range. After the screening of many nanoparticles, we obtained the best results with Fe2O3 NPs. In the future, we are planning to image a single intact cell by stigmatic type SALDI MS imaging using nanoparticles or nanostructured surfaces.