Abstract

Poster Presentations

Day 2, May 16（Thu.）　　Room P (Multi-purpose Hall)

2P-35　　PDF

Identification of Biomarkers and Therapeutic Targets of Multiple Sclerosis through MALDI-Imaging Mass Spectrometry based in situ Proteomics for the Central Nervous Systems (CNS) of Experimental Autoimmune Encephalomyelitis (EAE) Mouse Model

(¹Doshisha Univ., ²Bruker, ³Kyoto Pref. Univ. Med., ⁴Kansai Med. Univ.)
^oNami Tanaka¹, Hiroki Yamashita¹, Takashi Nirasawa², Ryo Kajita², Katsutoshi Taguchi³, Masaki Tanaka³, Takayuki Kondo⁴, Yudai Tsuji¹, Nobuto Kakuda¹, Masaya Ikegawa¹

Multiple sclerosis (MS) is an autoimmune disease its cardinal symptoms are chronic inflammatory, demyelination and axonal degeneration of central neuro system (CNS). In this study, we applied matrix assisted laser desorption/ionization (MALDI) imaging mass spectrometry (IMS) for　CNS of murine EAE without on-tissue digestion. We have successfully identified and visualized some specific proteins specifically expressed in lesions and investigated a candidate of therapeutic target. It was validated on an EAE model with or without blocking agents of the proteins. Furthermore, using LC-TIMS-MS/MS, in depth proteomics for the cells infiltrated into CNS was also performed.