日本質量分析学会 第67回質量分析総合討論会

Abstract

Poster Presentations

Day 2, May 16(Thu.)  Room P (Multi-purpose Hall)

Development of a SPE LC-MS/MS Method for the Bioanalytical Quantification of Pramlintide from Serum

(1Nihon Waters, 2Waters)
oMaki Terasaki1, Etsuko Suzuki1, Thanai Paxton1, C Dunning2, M Lame2, M Wrona2, K Haynes2

Pramlintide acetate (SYMLINTM) is a synthetic analogue of the human hormone amylin developed as an adjunctive therapy for patients with type 1 and 2 diabetes. With nearing patent expiry dates, and recent research indicating a role for amylin in Alzheimer’s Disease models, interest in amylin and amylin agonists is rising. Hydrophobic peptides such as pramlintide often suffer from non-specific binding (adsorption) to any labware samples come into contact with (plates, pipette tips, etc…). This can make method development difficult as it can lead to poor recovery, loss of analyte, and poor limits of detection. This work describes optimization and development of a selective sample preparation strategy and LC-MS/MS analysis to achieve LLOQs of 25 pg/mL from 100 μL of serum.