- Day 2, May 16（Thu.） 10:50-11:10 Room B (102)
A Novel Strategy for the Pathological Study of Alzheimer's Disease (AD) Brain with MALDI Imaging Mass Spectrometry in Combination with Shotgun Proteomics
Neuropathology of Alzheimer’s disease (AD) is characterized by the accumulation and aggregation of Amyloid β (Aβ) peptides into extracellular plaques of the brain. The Aβ peptides, composed of forty amino acids, are generated from amyloid precursor proteins (APP) by β- and γ-secretases. Aβ is deposited not only in cerebral parenchyma but also in leptomeningeal and cerebral vessel walls, known as cerebral amyloid angiopathy (CAA). While a variety of Aβ peptides were identified, detailed production and distribution of individual Aβ peptides in pathological tissues of AD and CAA is not fully addressed. Here, we develop a novel protocol of MALDI-imaging mass spectrometry (MALDI-IMS) in combination with shotgun proteomics on human autopsy brain tissues to obtain a comprehensive protein mapping.