日本質量分析学会 第67回質量分析総合討論会

Abstract

Oral Sessions

Day 1, May 15(Wed.) 16:25-16:45 Room B (102)

Detailed Structural Analysis of Lipids Using Hydrogen Attachment/Abstraction Dissociation (HAD) and Related Radical Induced Dissociation Techniques

(1Shimadzu, 2Doshisha Univ., 3AIST)
oHidenori Takahashi1, Yuji Shimabukuro2, Daiki Asakawa3, Shinichi Iwamoto1, Motoi Wada2, Koichi Tanaka1

Tandem mass spectrometry (MS/MS) is an effective analytical technique for the characterization of structurally diverse lipids. However, distinguishing isomeric lipids using the conventional collision-induced dissociation (CID) MS/MS technique often remains ambiguities, due to its lower reproducibly and specificity. To solve this limitation, a novel MS/MS technique of hydrogen attachment/abstraction dissociation (HAD) and oxygen attachment dissociation (OAD) was developed by our research group. In this study, we demonstrated the usefulness of HAD and OAD for double-bond position analysis of lipid and characterization of trans unsaturated fatty acids.