日本質量分析学会 第67回質量分析総合討論会

演題概要

オーラルセッション

第3日 5月17日(金) 14:45~15:05 C会場(101)

ネイティブ質量分析による蛋白質間および蛋白質-リガンド相互作用の親和性測定

(1阪大院工2エクセルズ)
o内山進1,2

We have been studying on protein-protein interactions and protein-ligand interactions as well as supramolecules composed of designed synthetic molecules by native mass spectrometry (native MS) and other biophysical methods such as analytical ultracentrifugation and isothermal titration calorimetry. In addition to stoichiometry of the interactions, estimations of thermodynamic and kinetic parameters are expected for the assessment of stability and dynamics of formed complexes. One approach to derive the quantity of each species based on native MS is analyzing the relative signal intensities of the free and bound states. In the presentation, examples of estimating quantitative interaction parameters in our researches and reports from other groups will be introduced followed by discussion about the pros and cons of native mass spectrometry.