日本質量分析学会 第66回質量分析総合討論会

Program

Poster Presentations

Day 4, May 18(Fri.)  Poster

Middle down analysis for histone H3 modification

(1Univ. Tokyo, 2Univ. Tokyo)
oYoko Chikaoka1, Hiroki Ueda2, Kazuki Yamamoto1, Sujin Lee1, Takeshi Kawamura1,2

Histones are highly modified molecules and their modifications are thought to be involved in epigenetic regulations and memory. Using Orbitrap Fusion, we have acquired high-resolution ETD spectra of histone H3.1 tail aa 1-49 peptides. We have found that the spectra have two main characteristics. First, the spectra had mixed ones of the positional isomers of modifications. Second, ETD for long peptides shows high frequencies of hydrogen rearrangements to provide 1-Da-gapped-clusters of ions. We are now doing efforts to resolve the mixed components and to improve charge determination using the hydrogen-rearranged clusters of ions.