日本質量分析学会 第66回質量分析総合討論会

Program

Oral Sessions

Day 4, May 18(Fri.) 14:45-15:05 Room B (Seiun 1)

TMT-based Quantitative Mass Spectrometry Reveals Proteomic Shifts between Traditional Hydrated Biofilm and DSB from Staphylococcus aureus: One Step Forward with Dry Surface Biofilm (DSB)

(1Macquarie Uni, 2APAF)
oMd Arifur Rahman1, Durdana Chowdhury1, Ardeshir Amirkhani2, Honghua Hu1, Mark Molloy2, Dana Pascovici2, Mark Baker1, Maria Mempin1, Xiaomin Song2, Karen Vickery1

Biofilms are problematic in healthcare settings, accounting for 65% of healthcare associated infections including indwelling medical devices, prostheses, etc. However, recent findings of biofilms on dry hospital surfaces emphasises the failures in current cleaning practices and disinfection. We aimed to determine how the protein component varies between traditional hydrated biofilms (wet) and DSB. S. aureus reference strain was grown in tryptic soy broth to produce a 24-hour planktonic culture, and over 12-days in the Centres for Disease Control biofilm reactor to produce the wet and DSB. TMT-based mass spectrometry (MS) was performed, and protein identification and relative quantitation of protein levels were performed using Proteome Discoverer (version 1.3). We identified 1636 total biofilm extractomes. Of these, 113 and 134 proteins showed significant abundance variation compared to planktonic bacteria in wet and DSB, respectively. In particular, UDP-N-acetylmuramate-L-alanine ligase (murC), is significantly up-regulated in DSB. This is involved in the peptidoglycan biosynthesis pathway, and is responsible for cell wall formation and might play a role in biofilm formation. This is the first report using high throughput TMT-based MS determining proteins in S. aureus. Current study will be helpful in designing advanced, targeted disinfectants and detergents to remove biofilms from dry environments.