Program

Poster Presentations

Day 3, May 17（Thu.）　　Poster

3P-09　　PDF

Evaluation of a peptide selection algorithm for trapped ion mobility with parallel accumulation serial fragmentation (TIMS PASEF) on a Q-TOF instrument timsTOF Pro with PASEF for shotgun proteomics

(¹Bruker Japan, ²Bruker Daltonik, ³Max Planck)
^oRyo Kajita¹, Markus Lubeck², Stephanie Kaspar-schoenefeld², Niels Goedecke², Oliver Raether², Nicole Drechsler², Michael Krause², Scarlet Koch², Heiner Koch², Florian Meier³, Jürgen Cox³, Matthias Mann³

The “Parallel Accumulation Serial Fragmentation” (PASEF) method for trapped ion mobility (TIMS) quadrupole time of flight (QTOF) instruments enables faster acquisition of fragment ion spectra from isolated precursor ions, without sacrificing spectral quality. During an ion mobility scan the quadrupole switches its isolation position several times, isolating a certain ion species only for the periods of time it actually elutes from the mobility separation device as a condensed package. A modified timsTOF instrument (Bruker) connected to a nanoElute UHPLC (Bruker) was used to test this approach for proteomics applications.