日本質量分析学会 第66回質量分析総合討論会

Program

Poster Presentations

Day 2, May 16(Wed.)  Poster

Establishment of method for peptidome analysis from a small amount of tissue

(1Kitasato Univ., 2Clin. Prot. Ctr., 3Kitasato Univ.)
oYuzuru Nakagawa1, Takuya Yamada1, Takaki Tsunoda1, Kazumi Fujimoto1,2, Makoto Itakura3, Yoshio Kodera1,2

The low-molecular-weight protein and peptide component of tissue includes members of several physiologically important classes, such as cytokines, chemokines, peptide hormones, and neuropeptide. However, method for comparative analysis of peptides intended for a small amount of sample is required to discover biomarker. In this study, we established a method to analyze peptides in detail from a small amount of tissues. The analysis enabled us to identify about 2,500 peptides derived from 921 proteins that include 13 hormone precursor proteins from only 3 mg of mouse brain whole lysate.