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- Day 2, May 16（Wed.） Poster
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2P-40（2B-O3-M-1720） PDF
In situ picolitre pressure-probe-electrospray-ionization mass spectrometry for monitoring single cell metabolites change
Single cell metabolomics, called picolitre pressure-probe-electrospray-ionization mass spectrometry (picoPPESI-MS), was used to directly examine the relationship between cytoplasmic streaming, cell metabolites, and cell water relations in intact tomato (Solanum lycopersicum L. ‘Micro-Tom’) trichome cells. Cytoplasmic streaming was monitored under the digital microscope. And, cell metabolites were determined by picoPPESI-MS immediately after cellular fluids were collected from the cells using a cell pressure-probe (CPP). When the plants were subjected to water stress, the water potential and turgor pressure both declined; however, the cytoplasmic streaming velocity was maintained throughout treatment. Metabolites in glycolysis in cytosol and Krebs cycle in mitochondria were accumulated significantly in response to the water stress for osmotic adjustment, which sustained cytoplasmic streaming under the stress conditions. Because the system allowed us to identify the source organelles of the metabolites in the collected cell fluids, we conclude that this analytical method is capable of sequentially determining organelle-specific metabolic changes in intact plant cells under a given stress condition.