日本質量分析学会 第66回質量分析総合討論会

Program

Poster Presentations

Day 2, May 16(Wed.)  Poster

Evaluation of Transmission Geometry Optical System for Imaging of Intracellular Molecules using Atmospheric Pressure Ionization Mass Spectrometry

(Osaka Univ.)
oHayato Kawai

In imaging mass spectrometry (IMS), the cellular scale resolution is needed for development of new drugs and pathological analysis. Although cellular level IMS is practically accomplished with a spatial resolution of 5–10 μm using a commercial instrument, the technology has still challenging problems, and spatial resolution less than 1 μm is required to observe intracellular molecules. Moreover, the biological samples such as cultured cells should be placed in the atmospheric pressure to maintain the alive condition. These problems are originated in the optical system, so this system called reflection geometry which is employed in most of all IMS instruments is not suitable for high spatial resolution IMS. Therefore, we developed the new one called transmission geometry optical system in which an objective lens was placed behind a transparent sample plate at the atmospheric pressure. In this system, it is easy to collect ions because of no obstacles and the optical component which has high numerical aperture can be employed to make small laser spot size. In this study, mass spectra of crystal violet (CV) using atmospheric pressure laser ionization were measured and the optical system was evaluated.