日本質量分析学会 第66回質量分析総合討論会

Program

Poster Presentations

Day 2, May 16(Wed.)  Poster

Metabolic profile of bile acids and lipids in fibrosis of NASH-model liver using MALDI Imaging at 20 μm spatial resolution

(1Shimadzu, 2Kyushu Univ., 3Mitsubishi Tanabe Pharm.)
oYuzo Yamazaki1, Shinichi Yamaguchi1, Tomonori Oshikawa1, Daisuke Miura2, Makoto Yamazaki3

Non-alcoholic steatohepatitis (NASH) is an advanced form of Non-alcoholic fatty liver disease (NAFLD), and is recognized to often progress to cirrhosis and hepatocellular carcinoma. A liver excised from an animal model raised to forcibly express NASH is quite useful to study the disease, because pathological findings of the organ are similar to the one from human patients. Whereas some metabolomics methods using LC- or GC-MS have been applied to the analysis of the organ, visualization of metabolites on pathological section is necessary to discuss disease states with relevance to various staining. We will present a metabolic profile of biochemical compounds in some pathological states, which is possibly related to a dynamics of the fibrosis in liver, by using MALDI imaging.