Oral Sessions
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Poster Presentations
（Day1, Day2, Day3, Day4）
Luncheon Seminars
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Poster Presentations
- Day 2, May 16（Wed.） Poster
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2P-04 PDF
Assessment of reproducibility and quantitative performance of SWATH-mass spectrometry of bovine serum proteins.
Intramuscular fat (IMF) content strongly affects the economic value of beef cattle in Japan. In order to develop serum biomarkers to diagnosis the IMF content, we are planning a large scale proteomic analysis of beef cattle serum using SWATH. In this study, we performed SWATH of bovine serum in a fixed window (FIX) and a variable window (VAR) methods, and compared their reproducibility and quantitative performance. Quantitative linearity was also evaluated using spiked-in peptides. Proteins in the bovine serum were trypsinized and the resulting peptides were analyzed. Up to 172 proteins were successfully quantified by SWATH. In the five-times SWATH measurements, 79.7%, and 66.6% of the proteins were quantified with CV < 10% in FIX and VAR, respectively, indicating the higher reproducibility of FIX. In the three-times SWATH measurements in the same day and with the same ion library, number of quantified proteins were not different between the methods, but number of quantified proteins with CV < 10% or 20% were larger in FIX. These results suggested that the FIX method is suitable for quantitative proteomics of bovine serum. Quantification of spiked-in peptides by FIX showed high quantitative linearity in the peptide amount range of 10 fmol to 10 pmol.