日本質量分析学会 第66回質量分析総合討論会

Program

Poster Presentations

Day 2, May 16(Wed.)  Poster

Proteomic Responses Reveal Signal Transduction and Cytoskeleton Rearrangement to Salinity Stress of Pistachio Rootstocks

(1FAMU, 2Tabriz Univ., 3QAU, 4Maebashi IT)
Ramesh Katam1, Mohammad Akbari1,2, Nasar Mehana2, Ghazala Mustafa3, oKatsumi Sakata4, Kala Johnson1

Pistacia vera L., cultivated in arid and semi-arid regions, is one of the most important nuts. The mechanisms underlying salinity tolerance in this plant is poorly understood. Five one-year-old pistachio rootstocks were treated with four saline water regimes for 100 days and physiological, biochemical and proteomic analysis were carried out. Salinity decreased total chlorophyll content and carotenoids in the leaves, and ascorbic acid and total soluble proteins in both leaves and roots. Total phenolic compounds, proline, glycine betaine, and H2O2 content increased in all rootstocks. Three different ion exclusion strategies were observed in rootstocks, (i) Na+ exclusion in UCB-1, (ii) Cl- exclusion in Badami, (iii) and similar concentrations of Na+ and Cl- in the leaves and roots of Ghazvini, Akbari and Kale-Ghouchi.

High throughput comparative proteomics of roots identified 153 more abundant and 69 low abundant proteins in UCB-1 and 340 more abundant and 18 low abundant proteins in Akbari. Majority identified proteins have the functions related to stress responsive proteins, signal transduction, cell wall and cytoskeleton metabolism. The data suggests a strong linkage of molecular mechanism with the physiological traits in the cultivars with various salt tolerances, and lead to further functional elucidation to improve salt tolerance.