日本質量分析学会 第66回質量分析総合討論会

Program

Oral Sessions

Day 2, May 16(Wed.) 17:50-18:10 Room C (Seiun 2)

Peptide and Glycan Mass Spectrometry Imaging as Diagnostic Tool in Cancer Research

(1FII, 2RAH)
oPeter Hoffmann1, Parul Mittal1, Georgia Arentz1, Manuela Klingler-hofmann1, Martin Oehler2

Imaging Mass Spectrometry (IMS) is typically used to determine the distribution of proteins in fresh frozen tissue. Tryptic Peptide and Glycan Imaging has some advantages over imaging of intact proteins. These include peptide level analysis provides the possibility for identification by matching accurate m/z and in situ MS/MS to high quality LC-MS/MS data obtained through digestion of relevant laser dissected tissue. Formalin-fixed paraffin embedded (FFPE) tissue can be analysed after antigen retrieval. A novel method for investigating tissue-specific N-linked glycans was recently developed by our group on FFPE murine kidney. We have used those methods to potentially make diagnostic decisions for patients with endometrial and ovarian cancer.
Here we present the latest developments within our group, including up-to-date methods for analysis of FFPE tissue (e.g. tryptic peptide and PNGase F Glycan), a method for linking LC-MS/MS data of peptides to MALDI-IMS data using internal calibrants as well as the generation of data for a MALDI-IMS patient and disease specific tryptic peptide database and the use of tissue micro arrays. We present that IMS spatially profiles glycoforms in tissue-specific regions, while through LC-ESI-MS/MS the corresponding glycol compositions are characterized. These methods are applied to endometrial and ovarian cancer FFPE tissues.