Oral Sessions
（Day1, Day2, Day3, Day4）
Poster Presentations
（Day1, Day2, Day3, Day4）
Luncheon Seminars
（Day1, Day2, Day3, Day4）
Poster Presentations
- Day 1, May 15（Tue.） Poster
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1P-35 PDF
Measurement of Peptide Solutions Containing Buffer or Salt in Atmospheric Pressure Continuous Flow Infrared Desorption Electrospray Ionization and comparison with ESI
Liquid chromatography/mass spectrometry (LC/MS) has been widely used for analyzing biomolecules in drug discovery research. However, buffer or salts in sample preparation largely decrease ionization efficiency in conventionally used electrospray ionization (ESI). In this study, infrared (IR) laser desorption electrospray ionization has been developed for proteins. Solutions of a peptide, angiotensin II, containing a buffer, Tris-HCl or salt, NaCl, were measured using this method or ESI, and ion signal intensities of the peptide were compared. As the results, it became clear that IR laser desorption electrospray ionization has higher tolerance to Tris-HCl and NaCl compared to ESI.