Oral Sessions
(Day1, Day2, Day3, Day4)
Poster Presentations
(Day1, Day2, Day3, Day4)
Luncheon Seminars
(Day1, Day2, Day3, Day4)
Poster Presentations
- Day 1, May 15(Tue.) Poster
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1P-06 PDF
Development of a comprehensive two-dimensional gas chromatography (GCxGC) method to profile complex lipid mixtures in human sebum
Human sebum is a mixture of non-polar lipids, mainly fatty acids, triglycerides, wax esters and squalene. Relationships between sebum composition and skin function/condition have been extensively studied because of their importance to skin health. However, the majority of sebum components remain uninterpreted due to the unsatisfactory separation power of conventional methods. In this study, we have developed a comprehensive two-dimensional gas chromatography (GCxGC) method combined with high-resolution quadrupole time-of-flight mass spectrometry (HR-QTOFMS) to increase the separation/identification power for sebum profiling. The optimized GCxGC method allowed the comprehensive profiling of sebum lipids from free fatty acids to triglycerides in 230 min. Especially, fatty acids were distinctively separated according to the number of carbon atoms, the number of double bonds, and the position of double bonds. Furthermore, oleic acid (C18:1n9cis) and its geometrical isomer, elaidic acid (C18:1n9trans), which is known to have different effects on skin, were successfully separated. We concluded that the developed method was a powerful tool to reveal the roles of unknown sebum lipids for healthy skin.